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Степень достоверности полученных результатов.
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801
Достоверность полученных данных основана на достаточном количестве выполненных исследований (1400 ультразвуковых исследований), на использовании достаточного числа современных диагностических методов и поверенного оборудования, на применении критериев доказательной медицины и экспертизе первичной документации, оформлявшейся на всех этапах исследования.
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802
Итоговые данные и результаты исследования соотносятся с целью работы и поставленными задачами.
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803
Личный вклад в диссертационную работу состоит в том, что автором проанализирована научная литература по изучаемой проблеме - как отечественная, так и зарубежная.
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804
Соискатель непосредственно принимала участие в ультразвуковых исследованиях органов малого таза, заполняла медицинскую документацию, осуществляла динамическое наблюдение за пациентами.
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805
Автором самостоятельно произведен сбор архивных данных, создание базы данных, также выполнена разработка дизайна исследования.
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806
Непосредственно автором получены результаты исследования, представленные в диссертационной работе, выполнена их статистическая обработка с последующим анализом, систематизацией и сравнением с литературными данными.
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807
Сформулированы выводы и разработаны практические рекомендации, внедренные в практику ГБУЗ «Самарский областной медицинский центр Династия», ГБУЗ СО «Самарская городская поликлиника № 3», Частного учреждения здравоохранения «Клиническая больница «РЖД – Медицина» г. Самары».
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808
Несмотря на десятилетия изучения регуляции трансляции у бактерий, остаётся актуальным вопрос о том, что именно позволяет конкретной мРНК обеспечить быструю инициацию и эффективную трансляцию.
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809
Данная работа посвящена изучению особенностей синтетических и природных 5'-нетранслируемых областей (5’-UTR) бактериальных мРНК, влияющих на эффективность трансляции, с помощью библиотек репортёрных конструкций.
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810
Применение метода Flow-seq, сочетающего проточную цитометрию с последующим секвенированием нового поколения, позволило провести анализ in vivo для библиотек репортёрных конструкций из более 24,5 тысяч различных вариантов 5’-UTR и определить функциональную значимость отдельных их участков.
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811
В итоге помимо всех известных детерминант, обеспечивающих увеличение уровня синтезируемого белка, определили ранее не описанные особенности 5’-UTR, способствующие увеличению эффективности трансляции: низкая доля остатков цитидина, AG-повторы, как новый энхансер трансляции, аддитивный эффект нескольких SD-мотивов, дополнительного старт-кодона.
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812
Результаты представляют интерес с фундаментальной точки зрения, более подробно раскрывая особенности сложного процесса трансляции, и проливают новый свет на возможности регуляции уровня биосинтеза белка с практической точки зрения при решении задач биотехнологии.
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813
Цель настоящего исследования - разработка научных и технологических основ изготовления металлических биполярных пластин для топливных элементов с протонобменной мембраной для мобильных систем энергопитания.
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814
Разработаны конструктивные и технологические решения изготовления металлических биполярных пластин для топливного элемента с протонобменной мембраной для мобильных систем энергопитания мощностью до 1,5 кВт, в том числе: разработана и запатентована конструкция металлической БП, обеспечивающей эффективное снабжение каждого мембранно-электродного бока (МЭБ) - единичного топливного элемента - необходимым количеством топлива и окислителя, и обладающей достаточным запасом прочности и жесткости для создания электрического контакта и герметизации анодного пространства топливного элемента.
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815
Подобраны и обоснованы технологические режимы обработки титана при изготовлении биполярных пластин.
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816
Разработан способ формования каналов катодной части БП путем пошаговой деформации заготовки холодным способом, которая позволяет снизить критическое истончение материала и величину остаточных механических напряжений в нем.
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817
Разработан и запатентован способ повышения коррозионной стойкости металлических биполярных пластин ПОМТЭ путем нанесения защитного покрытия на основе сложных олигомеров и полимеров, являющихся побочным продуктом переработки биомассы в 5-гидроксиметилфурфурол.
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818
Исследовано влияние условий эксплуатации в широком диапазоне изменения параметров окружающей среды, реализация которых возможна при эксплуатации беспилотных летательных аппаратов (БПЛА), на электрические характеристики и стабильность работы батареи ПОМТЭ с открытым катодом.
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819
Результаты диссертационного исследования использованы ООО НПП «Интор» при создании экспериментального образца технологической линии изготовления биполярных пластин для топливных элементов с протонобменной мембраной, предназначенных для энергообеспечения БПЛА.
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820
Объект исследований – 128 коллекционных образцов ярового ячменя.
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821
Цель исследования – выделить источники для селекции ярового ячменя на основе оценки коллекционных образцов различного эколого-географического происхождения по урожайности и комплексу селекционно-ценных признаков.
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822
Методики: «Методические указания по изучению мировой коллекции ячменя и овса» (2012) и Международный классификатор СЭВ рода Hordeum L. (1983), Кривченко В.И.
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823
и др.
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824
(2008), Родина Н.А., Ефремова З.Г.
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825
(1986), Афанасенко О.С.
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826
(2005), Тороп Е.А.
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827
и др., (2011), Удовенко В.Г.
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828
(1988), Лисицын Е.М.
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829
(2003), Омаров Д.С.
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830
(1975), В.А.
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831
Зыкин и др.
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832
(2011).
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833
Доспехов Б.А.
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834
(1985), Зайцев Г.Н.
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835
(1973), Плохинский Н.А.
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836
(1969).
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837
Оборудование: экспресс-анализатор INFRAMATIC 8620, аппарат ВИМС РКС-1.
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838
Выявлены новые источники хозяйственно ценных признаков.
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839
Получены новые знания о закономерностях изменчивости и взаимосвязи селекционно-ценных признаков.
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840
Создан новый исходный материал.
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841
Выводы и предложения могут быть использованы в научных учреждениях, занимающихся селекцией.
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842
Объект исследования: музейное дело в Омской области.
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843
Цель работы: выявление закономерностей и особенностей процесса становления и развития музейного дела в Омской области с 1930-х до начала 1990-х гг.
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844
Методы исследования: историко-сравнительный, историко-генетический, локально-исторический, историко-типологический.
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845
Полученные результаты и новизна: привлечены разнообразные источники по истории и деятельности музеев, часть источников впервые вводится в научный оборот либо впервые привлекается к решению проблемы исторической реконструкции процесса развития музейного дела в Омской области; уточнен количественный и профильный состав музеев музейной сети, особенности ее формирования на разных этапах; выявлены и детализированы данные о возникновении тех или иных музеев; показана локальная специфика развития музейного дела в Омской области в 1930-х ?
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846
начале 1990-х гг.
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847
; прослежены пути развития музейного дела с учетом влияния государственных и партийных структур разного уровня и личного вклада музейных сотрудников, общественных групп и энтузиастов музейного дела; систематизированы и обобщены источники и материалы по истории и основным направлениям музейной деятельности музеев Омской области; систематизированы и проанализированы статистические данные с количественными показателями обеспеченности музейными площадями, кадрами и по основным видам деятельности государственных музеев Омской области и их филиалов; реконструирован процесс формирования сети общественных музеев в Омской области с 1960-х гг.
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848
до начала 1990-гг.
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849
; разработана целостная концепция формирования музейной сети и музейного дела региона.
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850
Область применения: изучение региональной истории; разработка учебных курсов «История музейного дела России», «Грантовая деятельность в сфере культуры», «Менеджмент и маркетинг в музейной деятельности»; разработка экскурсий и музейных занятий, экскурсионных маршрутов.
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851
В эксперименте разработан метод замещения дефекта белочной оболочки полового члена с применением децеллюляризированной трупной артерии.
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852
В группах биомоделей было изучено влияние имплантированного графта децеллюляризированной трупной артерии на уровень С-реактивного белка в сыворотке крови (как маркера системного ответа).
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853
В исследовании экспериментальным путем проведено сравнение разных видов сосудистых матриксов для пластики дефекта белочной оболочки полового члена.
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854
Проведены исследования по двум направлениям, по первому направлению проведена оценка применимости децеллюляризированной трупной артерии человека в качестве материала для трансплантации.
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855
Во втором направление изучалось свойство трупной артерии после криконсервации с позиций дальнейшего использования в качестве материала для трансплантации.
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856
Полученные результаты свидетельствуют о потенциальной применимости децеллюляризированной трупной артерии в качестве материала аллотрансплантации.
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857
Было установлено что применение децеллюляризированной трупной артерии человека снижает риски послеоперационных воспалительных и тромботических осложнений.
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858
Установлено, что криоконсервация аллогенного сосудистого матрикса достоверно снижает сохранность коллагена I типа в биоматериале, ухудшает его биомеханические показатели (снижает значение модуля Юнга и предельного напряжения ).
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859
Экспериментально доказано, что криоконсервация однократным замораживанием в 5% декстране 30000-40000, 7.5% альбумине человека и 10% DMSO с последующим хранением при температуре -80 С. Применение в качестве консервантов глицерина и пропандиола приводит к некрозам, появлению пикнотических ядер и разволокнению соединительнотканного матрикса.
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860
Разработана и успешно апробирована в эксперименте модифицированная удлиняющая метолика хирургического лечения болезни Пейрони включающая иссечение бляшки и пластику дефекта децеллюляризированной трупной артерией человека.
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861
Имплантированный графт из децеллюляризированной трупной артерии существенно не изменяет уровень С - реактивного белка в сыворотке (как маркера системного ответа ), а следовательно, не вызывает выраженного системного ответа.
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862
При изучении морфологической трансформации децеллюляризированной трупной артерии после замещения дефекта белочной оболочки установлено, что последняя постепенно трансформируется в нормальную белочную оболочку, не вызывая фиброза.
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863
Работа посвящена обоснованию и разработке педагогической модели скоростно-силовой подготовки юных баскетболистов 14-15 лет с использованием упражнений в парах с дополнительным отягощением.
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864
Автором обоснованы факторы, определяющие необходимость использования упражнений в парах с дополнительным отягощением в процессе скоростно-силовой подготовки юных баскетболистов 14-15 лет.
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865
Им установлены наиболее эффективные средства, методы и формы скоростно-силовой подготовки юных баскетболистов.
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866
Обоснованы психолого-педагогические условия необходимые для применения упражнений в парах с дополнительным отягощением.
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867
Экспериментально доказано высокая эффективность разработанной педагогической модели скоростно-силовой подготовки юных баскетболистов 14-15 лет с использованием упражнений в парах с дополнительным отягощением.
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868
Цель исследования: Оптимизация восстановительного лечения пациентов с дисциркуляторной энцефалопатией III стадии пожилого и старческого возраста с применением когнитивного тренинга и низкочастотной импульсной магнитотерапии на фоне медикаментозной терапии.
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869
Задачи исследования: 1.
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870
Определить реабилитационный потенциал пациентов с дисциркуляторной энцефалопатией III стадии пожилого и старческого возраста на основе интегральной количественной оценки неврологического, интеллектуально-мнестического статуса, функциональной активности в повседневной жизни и критериев «Международной классификации функционирования, нарушений жизнедеятельности и здоровья».
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872
Оценить влияние адаптированного когнитивного тренинга на неврологический статус, интеллектуально-мнестические функции, функциональную активность в повседневной жизни и реабилитационный потенциал больных старшей возрастной группы с дисциркуляторной энцефалопатией III стадии на фоне медикаментозной терапии.
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873
3.
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874
Оценить влияние низкочастотной импульсной магнитотерапии и когнитивного тренинга на фоне медикаментозной терапии на неврологический статус, интеллектуально-мнестические функции, функциональную активность в повседневной жизни и реабилитационный потенциал больных старшей возрастной группы с дисциркуляторной энцефалопатией III стадии.
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875
4.
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876
Дать сравнительную оценку эффективности комплексного применения когнитивного тренинга и низкочастотной импульсной магнитотерапии на фоне медикаментозной терапии у пациентов с дисциркуляторной энцефалопатией III стадии.
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877
5.
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878
Проанализировать устойчивость лечебных эффектов комплексного применения когнитивного тренинга и низкочастотной импульсной магнитотерапии на фоне медикаментозной терапии пациентов с дисциркуляторной энцефалопатией III стадии через 3 месяца после лечения.
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879
Выводы 1.
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880
У пациентов пожилого и старческого возраст с ДЭ III ст.
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881
ведущие клинико-неврологические синдромы были представлены двигательными и когнитивными нарушениями в 100 %, психопатологическими расстройствами – в 91 %, псевдобульбарными – в 77%, пирамидными – в 75 %, атактическими – в 74 % и кохлеовестибулярными – в 58 %.
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882
У пациентов с ДЭ III стадии неврологические и когнитивные расстройства были умеренной и тяжелой степени, функциональная активность в повседневной жизни, по данным шкалы IADL, была снижена до умеренного (50 %) и низкого (50 %) уровней.
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883
2.
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884
Разработанный метод диагностики, включающий набор функциональных доменов МКФ и интегральную оценку неврологического, интеллектуально-мнестического статуса, повседневной активности пациентов, позволял оценить РП и влияние проводимой терапии у пациентов пожилого и старческого возраста с ДЭ III стадии.
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885
3.
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886
На основе количественной оценки, детализации неврологического, интеллектуально-мнестического статуса и повседневной активности пациентов выявлено, что 50% пациентов с ДЭ III стадии имели РП среднего уровня, что предполагало возможность частичного восстановления нарушенных категорий жизнедеятельности.
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887
4.
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888
Курсовое применение церебропротекторов (микроциркулянтов и ноотропов) у пациентов пожилого и старческого возраста с ДЭ III стадии не позволяло уменьшить когнитивные расстройства, но способствовало стабилизации интеллектуально-мнестических функций и неврологического статуса на протяжении шести месяцев.
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889
5.
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890
Когнитивный тренинг в комплексе с медикаментозной профилактической терапией продолжительностью три месяца у пациентов с ДЭ III стадии пожилого и старческого возраста способствовал повышению кратковременной памяти в 1,8 раза и долговременной памяти - в 1,6 раза, при этом тяжесть неврологических нарушений снижалась на 13%, а функциональная активность в повседневной жизни повышалась на 26%.
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892
Низкочастотная импульсная магнитотерапия в комплексе с КТ и медикаментозной профилактической терапией у пациентов с ДЭ III стадии способствовала улучшению кратковременной памяти в 1,5 раз, долговременной памяти в 1,8 раз, уменьшению выраженности неврологических нарушений на 19 % и повышению функциональной активности в повседневной жизни на 36 %.
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893
7.
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894
При анализе отдаленных результатов у пациентов с ДЭ III стадии, получавших низкочастотную импульсную МТ, КТ и медикаментозную терапию, через три месяца показатели интеллектуально-мнестического и неврологического статуса не отличались от параметров после завершенного курса восстановительного лечения и по сравнению с исходными значениям повысились показатели кратковременной памяти на 79%, долговременной памяти на 85 %, функциональной активности в повседневной жизни на 36 %, неврологические расстройства уменьшились на 19%, при этом реабилитационный потенциал улучшился в 1,8 раз.
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895
Через три месяца после курса КТ у пациентов с ДЭ III стадии достигнутые позитивные эффекты нивелировались: показатели кратковременной и долговременной памяти снизились на 89% и 66 % соответственно, ухудшились показатели неврологического статуса на 18%, функциональной независимости в повседневной активности - на 26%.
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896
Объект исследования – процесс обучения стажёров из КНР публицистическому стилю речи с использованием интернет-ресурсов на занятиях по русскому языку как иностранному.
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897
Цель исследования - разработать комплекс упражнений и методических приёмов для обучения публицистическому стилю речи китайских стажёров на занятиях по РКИ на основе применения современных русскоязычных интернет-ресурсов.
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898
Теоретическая значимость исследования заключается в систематизации материалов в области публицистического регистра текстов, выведенных критериев отбора учебного материала для обучения публицистическому стилю речи с использованием интернет-ресурсов в курсе РКИ.
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899
Практическая значимость настоящего исследования заключается в разработке комплекса упражнений и методических приёмов для обучения публицистическому стилю речи с использованием интернет-ресурсов в курсе РКИ.
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