Datasets:

siddharthtumre

/

Revised-JNLPBA

like 0

[ "By", "using", "transient", "transfection", "assays", ",", "we", "found", "that", "cross-linking", "CD40", "supports", "NF-kappa", "B", "-dependent", "gene", "expression", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 1, 2, 2, 2, 0, 0 ]

[ "Our", "results", "define", "the", "NF-kappa", "B", "system", "as", "an", "intermediate", "event", "in", "CD40", "signaling", "and", "suggest", "that", "the", "CD40", "pathway", "can", "influence", "the", "expression", "of", "B", "cell-associated", "genes", "with", "NF-kappa", "B", "consensus", "sites", "." ]

[ 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0 ]

[ "Protease", "inhibitors", "block", "lipopolysaccharide", "induction", "of", "tissue", "factor", "gene", "expression", "in", "human", "monocytic", "cells", "by", "preventing", "activation", "of", "c-Rel/p65", "heterodimers", "." ]

[ 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 7, 8, 8, 0, 0, 0, 0, 9, 10, 0 ]

[ "Tissue", "factor", "(", "TF", ")", "is", "expressed", "rapidly", "by", "human", "monocytes", "exposed", "to", "bacterial", "endotoxin", "(", "lipopolysaccharide", ",", "or", "LPS", ")", "." ]

[ 9, 10, 0, 9, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Transcriptional", "regulation", "is", "mediated", "by", "binding", "of", "c-Rel/p65", "heterodimers", "to", "a", "kappa", "B-like", "site", "in", "the", "TF", "promoter", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 1, 2, 2, 0, 0, 1, 2, 0 ]

[ "Nuclear", "translocation", "of", "cytosolic", "c-Rel/p65", "heterodimers", "and", "other", "members", "of", "the", "NF-kappa", "B/Rel", "family", "requires", "dissociation", "and", "proteolytic", "degradation", "of", "the", "inhibitor", "protein", ",", "I", "kappa", "B", "alpha", "." ]

[ 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0 ]

[ "The", "protease", "inhibitors", "N", "alpha-tosylphenylalanyl", "chloromethyl", "ketone", "(", "TPCK", ")", "and", "N", "alpha-tosyl-L-lysine", "chloromethyl", "ketone", "(", "TLCK", ")", "block", "activation", "of", "NF-kappa", "B/Rel", "proteins", "by", "preventing", "degradation", "of", "I", "kappa", "B", "alpha", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 9, 10, 10, 10, 0 ]

[ "To", "determine", "if", "TPCK", "and", "TLCK", "inhibited", "LPS", "induction", "of", "TF", "expression", ",", "freshly", "isolated", "human", "monocytes", "and", "monocytic", "THP-1", "cells", "were", "pretreated", "with", "these", "inhibitors", "for", "30", "min", "before", "LPS", "stimulation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 7, 8, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Both", "TPCK", "and", "TLCK", "inhibited", "LPS", "induction", "of", "TF", "protein", ",", "TF", "mRNA", "and", "TF", "promoter", "activity", "in", "a", "dose-dependent", "manner", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 3, 4, 0, 1, 2, 0, 0, 0, 0, 0, 0 ]

[ "These", "inhibitors", "specifically", "prevented", "degradation", "of", "I", "kappa", "B", "alpha", "and", "nuclear", "translocation", "of", "c-Rel/p65", "heterodimers", "." ]

[ 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 9, 10, 0 ]

[ "In", "contrast", ",", "TPCK", "and", "TLCK", "did", "not", "block", "induction", "of", "an", "immediate-early", "gene", "encoding", "the", "transcription", "factor", ",", "Egr-1", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]

[ "Taken", "together", ",", "these", "data", "indicated", "that", "inhibiting", "nuclear", "translocation", "of", "c-Rel/p65", "heterodimers", "prevented", "LPS", "induction", "of", "TF", "gene", "transcription", "in", "monocytic", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 1, 2, 0, 0, 7, 8, 0 ]

[ "1", ",", "25-Dihydroxyvitamin", "D3", "receptors", "in", "peripheral", "blood", "mononuclear", "cells", "from", "patients", "with", "primary", "and", "secondary", "hyperparathyroidism", "." ]

[ 9, 10, 10, 10, 10, 0, 7, 8, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "A", "decreased", "number", "of", "calcitriol", "(", "1", ",", "25", "(", "OH", ")", "2D3", ")", "receptors", "has", "been", "observed", "in", "parathyroid", "glands", "of", "uremic", "animals", "." ]

[ 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "In", "humans", ",", "studies", "carried", "out", "in", "surgically", "removed", "parathyroid", "glands", "have", "shown", "that", "calcitriol", "binding", "is", "higher", "in", "primary", "than", "in", "secondary", "hyperparathyroidism", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Since", "specific", "receptors", "for", "calcitriol", "have", "been", "described", "in", "peripheral", "blood", "mononuclear", "cells", "(", "PBMC", ")", ",", "we", "have", "investigated", "the", "specific", "uptake", "of", "3H-labelled", "1", ",", "25", "(", "OH", ")", "2D3", "in", "PBMC", "of", "12", "women", "with", "primary", "hyperparathyroidism", "(", "PHP", ")", ",", "8", "women", "with", "hyperparathyroidism", "secondary", "to", "chronic", "renal", "failure", "(", "SH", ")", ",", "9", "women", "with", "renal", "transplant", "(", "RT", ")", ",", "and", "23", "healthy", "women", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 8, 8, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "median", "dissociation", "constant", "(", "Kd", ")", "was", "similar", "in", "all", "three", "groups", "of", "patients", "and", "in", "healthy", "women", "(", "mean", "+/-", "S.D.", "(", "range", ")", ":", "PHP", ",", "1.2", "+/-", "1.0", "(", "0.2-4", ")", "x", "10", "(", "-10", ")", "M", ";", "SH", ",", "0.6", "+/-", "0.4", "(", "0.2-1.2", ")", "x", "10", "(", "-10", ")", "M", ";", "RT", ",", "1.1", "+/-", "0.5", "(", "0.4-1.9", ")", "x", "10", "(", "-10", ")", "M", ";", "controls", ",", "1.0", "+/-", "0.6", "(", "0.3-2.6", ")", "x", "10", "(", "-10", ")", "M", ")", ".", "However", ",", "the", "maximal", "binding", "capacity", "(", "Nmax", ")", "was", "significantly", "enhanced", "in", "PHP", "(", "3.9", "+/-", "1.9", "(", "1.3-7.6", ")", "fmol/10", "(", "7", ")", "cells", "vs.", "2.3", "+/-", "0.9", "(", "1.1-4.4", ")", "fmol/10", "(", "7", ")", "cells", "in", "controls", ";", "P", "=", "0.0006", ")", "and", "decreased", "in", "SH", "(", "0.8", "+/-", "0.5", "(", "0.2-1.6", ")", "fmol/10", "(", "7", ")", "cells", "vs.", "2.3", "+/-", "0.9", "(", "1.1-4.4", ")", "fmol/10", "(", "7", ")", "cells", "in", "controls", ";", "P", "=", "0.0001", ")", ",", "whereas", "no", "changes", "were", "seen", "in", "RT", "(", "2.3", "+/-", "0.7", "(", "1.2-3.3", ")", "fmol/10", "(", "7", ")", "cells", "vs.", "2.3", "+/-", "0.9", "(", "1.1-4.4", ")", "fmol/10", "(", "7", ")", "cells", "in", "controls", ")", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "In", "three", "patients", "with", "PHP", "who", "were", "subjected", "to", "parathyroidectomy", ",", "the", "calcitriol", "number", "came", "down", "to", "normal", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Changes", "of", "calcitriol", "receptors", "in", "primary", "and", "secondary", "hyperparathyroidism", "could", "magnify", "the", "consequences", "of", "disturbances", "in", "serum", "concentration", "of", "calcitriol", "itself", "and", "might", "play", "an", "important", "role", "in", "the", "development", "of", "secondary", "hyperparathyroidism", "in", "uremia", "." ]

[ 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "severe", "phenotype", "of", "females", "with", "tiny", "ring", "X", "chromosomes", "is", "associated", "with", "inability", "of", "these", "chromosomes", "to", "undergo", "X", "inactivation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Mental", "retardation", "and", "a", "constellation", "of", "congenital", "malformations", "not", "usually", "associated", "with", "Turner", "syndrome", "are", "seen", "in", "some", "females", "with", "a", "mosaic", "45", ",", "X/46", ",", "X", ",", "r", "(", "X", ")", "karyotype", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Studies", "of", "these", "females", "show", "that", "the", "XIST", "locus", "on", "their", "tiny", "ring", "X", "chromosomes", "is", "either", "not", "present", "or", "not", "expressed", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "As", "XIST", "transcription", "is", "well", "correlated", "with", "inactivation", "of", "the", "X", "chromosome", "in", "female", "somatic", "cells", "and", "spermatogonia", ",", "nonexpression", "of", "the", "locus", "even", "when", "it", "is", "present", "suggests", "that", "these", "chromosomes", "are", "transcriptionally", "active", "." ]

[ 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "We", "examined", "the", "transcriptional", "activity", "of", "ring", "X", "chromosomes", "lacking", "XIST", "expression", "(", "XISTE-", ")", ",", "from", "three", "females", "with", "severe", "phenotypes", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "two", "tiny", "ring", "X", "chromosomes", "studied", "with", "an", "antibody", "specific", "for", "the", "acetylated", "isoforms", "of", "histone", "H4", "marking", "transcribed", "chromatin", "domains", "were", "labeled", "at", "a", "level", "consistent", "with", "their", "being", "active", "." ]

[ 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "We", "also", "examined", "tow", "of", "the", "XISTE-", "ring", "chromosomes", "to", "determine", "whether", "genes", "that", "are", "normally", "silent", "on", "an", "inactive", "X", "are", "expressed", "from", "these", "chromosomes", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Analyses", "of", "hybrid", "cells", "show", "that", "TIMP", ",", "ZXDA", ",", "and", "ZXDB", "loci", "on", "the", "proximal", "short", "arm", ",", "and", "AR", "and", "PHKA1", "loci", "on", "the", "long", "arm", ",", "are", "well", "expressed", "from", "the", "tiny", "ring", "X", "chromosome", "lacking", "XIST", "DNA", "." ]

[ 0, 0, 0, 0, 0, 0, 1, 0, 1, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 1, 2, 0 ]

[ "Studies", "of", "the", "ring", "chromosome", "that", "has", "XIST", "DNA", "but", "does", "not", "transcribe", "it", "show", "that", "its", "AR", "allele", "is", "transcribed", "along", "with", "the", "one", "on", "the", "normal", "X", "allele", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "(", "ABSTRACT", "TRUNCATED", "AT", "250", "WORDS", ")" ]

[ 0, 0, 0, 0, 0, 0, 0 ]

[ "Inhibition", "of", "activation", "of", "transcription", "factor", "AP-1", "by", "CD28", "signalling", "in", "human", "T-cells", "." ]

[ 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 7, 8, 0 ]

[ "Co-stimulation", "of", "T-lymphocytes", "by", "T-cell", "receptor", "(", "TcR", ")", "occupancy", "and", "activation", "of", "the", "CD28", "surface", "molecule", "results", "in", "enhanced", "proliferation", "and", "interleukin", "2", "(", "IL-2", ")", "production", "." ]

[ 0, 0, 7, 0, 9, 10, 0, 9, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 9, 10, 0, 9, 0, 0, 0 ]

[ "The", "increase", "in", "IL-2", "gene", "expression", "triggered", "by", "CD28", "involves", "a", "kappa", "B-like", "sequence", "in", "the", "5'-regulatory", "region", "of", "the", "IL-2", "promoter", ",", "called", "CD28-responsive", "element", "." ]

[ 0, 0, 0, 1, 2, 0, 0, 0, 9, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0 ]

[ "Stimulation", "of", "T-cells", "by", "agonistic", "anti-CD28", "antibodies", "in", "conjunction", "with", "phorbol", "12-myristate", "13-acetate", "(", "PMA", ")", "-", "or", "TcR", "-derived", "signals", "induces", "the", "enhanced", "activation", "of", "the", "transcription", "factor", "NF-kappa", "B", "." ]

[ 0, 0, 7, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0 ]

[ "Here", "we", "report", "that", "CD28", "engagement", ",", "however", ",", "exerts", "opposite", "effects", "on", "the", "transcription", "factor", "AP-1", "." ]

[ 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0 ]

[ "Whereas", "anti-CD28", "together", "with", "PMA", "increased", "the", "DNA", "binding", "and", "trans-activation", "activity", "of", "NF-kappa", "B", ",", "PMA-induced", "activation", "of", "AP-1", "was", "significantly", "suppressed", "." ]

[ 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 9, 0, 0, 0, 0 ]

[ "The", "inhibitory", "effect", "exerted", "by", "anti-CD28", "was", "observed", "at", "the", "level", "of", "DNA", "binding", "as", "well", "as", "in", "functional", "reporter-gene", "assays", "." ]

[ 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "These", "results", "suggest", "that", "the", "two", "transcription", "factors", "are", "independently", "regulated", "and", "may", "perform", "different", "functions", "during", "T-cell", "activation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0 ]

[ "HIV-1", "Nef", "leads", "to", "inhibition", "or", "activation", "of", "T", "cells", "depending", "on", "its", "intracellular", "localization", "." ]

[ 9, 10, 0, 0, 0, 0, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0 ]

[ "Nef", "of", "primate", "lentiviruses", "is", "required", "for", "viremia", "and", "progression", "to", "AIDS", "in", "monkeys", "." ]

[ 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Negative", ",", "positive", ",", "and", "no", "effects", "of", "Nef", "have", "also", "been", "reported", "on", "viral", "replication", "in", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "To", "reconcile", "these", "observations", ",", "we", "expressed", "a", "hybrid", "CD8-Nef", "protein", "in", "Jurkat", "cells", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 5, 6, 0 ]

[ "Two", "opposite", "phenotypes", "were", "found", ",", "which", "depended", "on", "the", "intracellular", "localization", "of", "Nef", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]

[ "Expressed", "in", "the", "cytoplasm", "or", "on", "the", "cell", "surface", ",", "the", "chimera", "inhibited", "or", "activated", "early", "signaling", "events", "from", "the", "T", "cell", "antigen", "receptor", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0 ]

[ "Activated", "Jurkat", "cells", "died", "by", "apoptosis", ",", "and", "only", "cells", "with", "mutated", "nef", "genes", "expressing", "truncated", "Nefs", "survived", ",", "which", "rendered", "Nef", "nonfunctional", "." ]

[ 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 9, 0, 0, 0, 0, 9, 0, 0 ]

[ "These", "mutations", "paralleled", "those", "in", "other", "viral", "strains", "passaged", "in", "vitro", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Not", "only", "do", "these", "positional", "effects", "of", "Nef", "reconcile", "diverse", "phenotypes", "of", "Nef", "and", "suggest", "a", "role", "for", "its", "N-terminal", "myristylation", ",", "but", "they", "also", "explain", "effects", "of", "Nef", "in", "HIV", "infection", "and", "progression", "to", "AIDS", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "An", "intricate", "arrangement", "of", "binding", "sites", "for", "the", "Ets", "family", "of", "transcription", "factors", "regulates", "activity", "of", "the", "alpha", "4", "integrin", "gene", "promoter", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 0 ]

[ "alpha", "4", "integrins", "mediate", "cell-cell", "and", "cell-extracellular", "matrix", "interactions", "that", "are", "critical", "for", "maturation", "and", "function", "of", "the", "immune", "system", "as", "well", "as", "differentiation", "of", "skeletal", "muscle", "." ]

[ 9, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Here", "we", "examine", "molecular", "mechanisms", "controlling", "the", "pattern", "of", "alpha", "4", "expression", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "activity", "of", "constructs", "containing", "5", "'", "deletion", "mutants", "of", "the", "alpha", "4", "gene", "promoter", "was", "compared", "in", "transfection", "assays", "into", "cell", "lines", "that", "express", "alpha", "4", "and", "cell", "lines", "that", "do", "not", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "The", "sequence", "between", "position", "-42", "and", "-76", "base", "pairs", "(", "bp", ")", "was", "required", "for", "efficient", "transcription", "in", "cells", "that", "express", "alpha", "4", ",", "but", "it", "showed", "no", "activity", "in", "HeLa", "cells", ",", "which", "do", "not", "express", "alpha", "4", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Three", "binding", "sites", "for", "the", "Ets", "family", "of", "transcription", "factors", "are", "found", "in", "this", "region", ":", "two", "adjacent", "sites", "at", "positions", "-50", "and", "-54", "bp", "and", "a", "more", "5", "'", "site", "at", "position", "-67", "bp", "." ]

[ 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Using", "a", "series", "of", "constructs", "containing", "deletions", "and", "mutations", "in", "this", "region", ",", "we", "found", "that", "the", "3'-most", "site", "alone", "was", "sufficient", "for", "binding", "GA-binding", "protein", "alpha", "(", "GABP", "alpha", ")", "/GABP", "beta", "and", "for", "a", "low", "level", "of", "transcriptional", "activation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 10, 10, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "When", "all", "three", "sites", "were", "present", ",", "a", "second", "complex", "``", "a", "''", "was", "detected", ",", "which", "contains", "an", "unknown", "member", "of", "the", "Ets", "family", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0 ]

[ "Formation", "of", "complex", "a", "was", "cell-type", "specific", "and", "correlated", "with", "a", "high", "level", "of", "transcription", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Deletion", "of", "the", "5'-most", "Ets", "site", "had", "no", "effect", "on", "binding", "to", "GABP", "alpha/GABP", "beta", ",", "but", "it", "eliminated", "a", "." ]

[ 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0 ]

[ "Concomitant", "with", "this", "loss", "of", "a", ",", "a", "new", "Ets-1-containing", "complex", "``", "c", "``", "appeared", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0 ]

[ "Complex", "c", "substituted", "efficiently", "for", "complex", "a", "in", "transcriptional", "activation", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "We", "conclude", "that", "although", "neither", "of", "the", "two", "5'-most", "Ets", "sites", "alone", "binds", "nuclear", "protein", ",", "they", "appear", "to", "act", "as", "modulators", "which", "control", "the", "pattern", "of", "Ets", "proteins", "that", "bind", "the", "alpha", "4", "gene", "promoter", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 1, 2, 2, 2, 0 ]

[ "This", "arrangement", "of", "Ets", "sites", ",", "coupled", "with", "the", "tissue-", "and", "developmental-specific", "expression", "of", "Ets", "members", ",", "likely", "play", "a", "key", "role", "in", "defining", "the", "pattern", "of", "alpha", "4", "integrin", "." ]

[ 0, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0 ]

[ "Mechanism", "of", "antiandrogen", "action", ":", "conformational", "changes", "of", "the", "receptor", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Androgen", "receptor", "mRNA", "was", "translated", "in", "vitro", ",", "and", "androgen-", "and", "antiandrogen-bound", "receptor", "complexes", "were", "studied", "using", "limited", "proteolytic", "digestion", "by", "trypsin", "." ]

[ 3, 4, 4, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0 ]

[ "Partial", "proteolysis", "of", "androgen-bound", "receptor", "protein", "resulted", "in", "a", "29-kDa", "proteolysis-resisting", "fragment", ",", "whereas", "antiandrogen", "binding", "stabilised", "a", "35-kDa", "fragment", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Both", "fragments", "contain", "the", "entire", "ligand", "binding", "domain", ",", "and", "the", "35-kDa", "fragment", "extended", "into", "the", "hinge", "region", "of", "the", "receptor", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Several", "antiandrogens", "show", "agonistic", "properties", "for", "a", "mutated", "androgen", "receptor", "(", "LNCaP", "cell", "variant", ")", ";", "trypsin", "digestion", "of", "antiandrogen-bound", "mutated", "receptor", "also", "resulted", "in", "a", "29-kDa", "fragment", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 5, 6, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Our", "results", "point", "to", "an", "important", "difference", "between", "antiandrogens", "and", "antagonists", "of", "other", "steroid", "hormone", "receptors", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0 ]

[ "Antiandrogens", "result", "in", "protection", "of", "both", "the", "hinge", "region", "and", "C-terminus", "of", "the", "androgen", "receptor", "agonist", "proteolytic", "attack", ",", "whereas", "other", "studies", "showed", "that", "antiestrogens", "and", "antiprogestagens", "expose", "the", "C-terminal", "end", "of", "the", "ligand", "binding", "domain", "of", "their", "respective", "receptors", "to", "protease", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Differences", "in", "conformation", "of", "the", "hinge", "region", "distinguish", "androgen-bound", "from", "antiandrogen-bound", "receptor", "complexes", ",", "which", "represents", "an", "important", "feature", "of", "antiandrogen", "action", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Activation", "of", "nuclear", "factor", "kappa", "B", "in", "human", "neuroblastoma", "cell", "lines", "." ]

[ 0, 0, 9, 10, 10, 10, 0, 5, 6, 6, 6, 0 ]

[ "The", "nuclear", "factor", "kappa", "B", "(", "NF-kappa", "B", ")", "is", "a", "eukaryotic", "transcription", "factor", "." ]

[ 0, 9, 10, 10, 10, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0 ]

[ "In", "B", "cells", "and", "macrophages", "it", "is", "constitutively", "present", "in", "cell", "nuclei", ",", "whereas", "in", "many", "other", "cell", "types", ",", "NF-kappa", "B", "translocates", "from", "cytosol", "to", "nucleus", "as", "a", "result", "of", "transduction", "by", "tumor", "necrosis", "factor", "alpha", "(", "TNF", "alpha", ")", ",", "phorbol", "ester", ",", "and", "other", "polyclonal", "signals", "." ]

[ 0, 7, 8, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Using", "neuroblastoma", "cell", "lines", "as", "models", ",", "we", "have", "shown", "that", "in", "neural", "cells", "NF-kappa", "B", "was", "present", "in", "the", "cytosol", "and", "translocated", "into", "nuclei", "as", "a", "result", "of", "TNF", "alpha", "treatment", "." ]

[ 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 7, 8, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0 ]

[ "The", "TNF", "alpha", "-activated", "NF-kappa", "B", "was", "transcriptionally", "functional", "." ]

[ 0, 9, 10, 0, 9, 10, 0, 0, 0, 0 ]

[ "NF-kappa", "B", "activation", "by", "TNF", "alpha", "was", "not", "correlated", "with", "cell", "differentiation", "or", "proliferation", "." ]

[ 9, 10, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "However", ",", "reagents", "such", "as", "nerve", "growth", "factor", "(", "NGF", ")", "and", "the", "phorbol", "ester", "phorbol", "12-myristate", "13-acetate", "(", "PMA", ")", ",", "which", "induce", "phenotypical", "differentiation", "of", "the", "SH-SY5Y", "neuroblastoma", "cell", "line", ",", "activated", "NF-kappa", "B", ",", "but", "only", "in", "that", "particular", "cell", "line", "." ]

[ 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "In", "a", "NGF-responsive", "rat", "pheochromocytoma", "cell", "line", ",", "PC12", ",", "PMA", "activated", "NF-kappa", "B", ",", "whereas", "NGF", "did", "not", "." ]

[ 0, 0, 0, 5, 6, 6, 6, 0, 5, 0, 0, 0, 9, 10, 0, 0, 9, 0, 0, 0 ]

[ "In", "other", "neuroblastoma", "cell", "lines", ",", "such", "as", "SK-N-Be", "(", "2", ")", ",", "the", "lack", "of", "PMA", "induction", "of", "differentiation", "was", "correlated", "with", "the", "lack", "of", "NF-kappa", "B", "activation", "." ]

[ 0, 0, 5, 6, 6, 0, 0, 0, 5, 6, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0 ]

[ "We", "found", ",", "moreover", ",", "that", "in", "SK-N-Be", "(", "2", ")", "cells", "protein", "kinase", "C", "(", "PKC", ")", "enzymatic", "activity", "was", "much", "lower", "compared", "with", "that", "in", "a", "control", "cell", "line", "and", "that", "the", "low", "PKC", "enzymatic", "activity", "was", "due", "to", "low", "PKC", "protein", "expression", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 6, 6, 9, 10, 10, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 9, 10, 0, 0 ]

[ "NF-kappa", "B", "was", "not", "activated", "by", "retinoic", "acid", ",", "which", "induced", "morphological", "differentiation", "of", "all", "the", "neuroblastoma", "cell", "lines", "used", "in", "the", "present", "study", "." ]

[ 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0 ]

[ "Thus", ",", "NF-kappa", "B", "activation", "was", "not", "required", "for", "neuroblastoma", "cell", "differentiation", "." ]

[ 0, 0, 9, 10, 0, 0, 0, 0, 0, 7, 8, 0, 0 ]

[ "Furthermore", ",", "the", "results", "obtained", "with", "TNF", "alpha", "proved", "that", "NF-kappa", "B", "activation", "was", "not", "sufficient", "for", "induction", "of", "neuroblastoma", "differentiation", "." ]

[ 0, 0, 0, 0, 0, 0, 9, 10, 0, 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "ERP", ",", "a", "new", "member", "of", "the", "ets", "transcription", "factor/oncoprotein", "family", ":", "cloning", ",", "characterization", ",", "and", "differential", "expression", "during", "B-lymphocyte", "development", "." ]

[ 9, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0 ]

[ "The", "ets", "gene", "family", "encodes", "a", "group", "of", "proteins", "which", "function", "as", "transcription", "factors", "under", "physiological", "conditions", "and", ",", "if", "aberrantly", "expressed", ",", "can", "cause", "cellular", "transformation", "." ]

[ 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "We", "have", "recently", "identified", "two", "regulatory", "elements", "in", "the", "murine", "immunoglobulin", "heavy-chain", "(", "IgH", ")", "enhancer", ",", "pi", "and", "microB", ",", "which", "exhibit", "striking", "similarity", "to", "binding", "sites", "for", "ets-related", "proteins", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0, 1, 0, 1, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 0 ]

[ "To", "identify", "ets-related", "transcriptional", "regulators", "expressed", "in", "pre-B", "lymphocytes", "that", "may", "interact", "with", "either", "the", "pi", "or", "the", "microB", "site", ",", "we", "have", "used", "a", "PCR", "approach", "with", "degenerate", "oligonucleotides", "encoding", "conserved", "sequences", "in", "all", "members", "of", "the", "ets", "family", "." ]

[ 0, 0, 9, 10, 10, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 1, 0, 0, 1, 2, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 0 ]

[ "We", "have", "cloned", "the", "gene", "for", "a", "new", "ets-related", "transcription", "factor", ",", "ERP", "(", "ets-related", "protein", ")", ",", "from", "the", "murine", "pre-B", "cell", "line", "BASC", "6C2", "and", "from", "mouse", "lung", "tissue", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 9, 10, 0, 0, 0, 0, 5, 6, 6, 6, 6, 6, 0, 0, 0, 0, 0, 0 ]

[ "The", "ERP", "protein", "contains", "a", "region", "of", "high", "homology", "with", "the", "ETS", "DNA-binding", "domain", "common", "to", "all", "members", "of", "the", "ets", "transcription", "factor/oncoprotein", "family", "." ]

[ 0, 9, 10, 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 0, 0, 0, 0, 0, 9, 10, 10, 10, 0 ]

[ "Three", "additional", "smaller", "regions", "show", "homology", "to", "the", "ELK-1", "and", "SAP-1", "genes", ",", "a", "subgroup", "of", "the", "ets", "gene", "family", "that", "interacts", "with", "the", "serum", "response", "factor", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 1, 0, 1, 2, 0, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 9, 10, 10, 0 ]

[ "Full-length", "ERP", "expresses", "only", "negligible", "DNA-binding", "activity", "by", "itself", "." ]

[ 0, 9, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Removal", "of", "the", "carboxy", "terminus", "enables", "ERP", "to", "interact", "with", "a", "variety", "of", "ets-binding", "sites", "including", "the", "E74", "site", ",", "the", "IgH", "enhancer", "pi", "site", ",", "and", "the", "lck", "promoter", "ets", "site", ",", "suggesting", "a", "carboxy-terminal", "negative", "regulatory", "domain", "." ]

[ 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 1, 2, 0, 0, 1, 2, 0, 0, 1, 2, 2, 2, 0, 0, 0, 1, 2, 2, 2, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "At", "least", "three", "ERP-related", "transcripts", "are", "expressed", "in", "a", "variety", "of", "tissues", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "However", ",", "within", "the", "B-cell", "lineage", ",", "ERP", "is", "highly", "expressed", "primarily", "at", "early", "stages", "of", "B-lymphocyte", "development", ",", "and", "expression", "declines", "drastically", "upon", "B-cell", "maturation", ",", "correlating", "with", "the", "enhancer", "activity", "of", "the", "IgH", "pi", "site", "." ]

[ 0, 0, 0, 0, 7, 8, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 7, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0 ]

[ "These", "data", "suggest", "that", "ERP", "might", "play", "a", "role", "in", "B-cell", "development", "and", "in", "IgH", "gene", "regulation", "." ]

[ 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 7, 0, 0, 0, 1, 2, 0, 0 ]

[ "Gene", "for", "a", "tissue-specific", "transcriptional", "activator", "(", "EBF", "or", "Olf-1", ")", ",", "expressed", "in", "early", "B", "lymphocytes", ",", "adipocytes", ",", "and", "olfactory", "neurons", ",", "is", "located", "on", "human", "chromosome", "5", ",", "band", "q34", ",", "and", "proximal", "mouse", "chromosome", "11", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 9, 0, 9, 0, 0, 0, 0, 0, 7, 8, 0, 7, 0, 0, 7, 8, 0, 0, 0, 0, 1, 2, 2, 0, 0, 1, 0, 0, 0, 1, 2, 2, 0 ]

[ "Murine", "B", "lymphocytes", ",", "adipocytes", ",", "and", "olfactory", "neurons", "contain", "a", "DNA-binding", "protein", "that", "participates", "in", "the", "regulation", "of", "genes", "encoding", "tissue-specific", "components", "of", "signal", "transduction", "." ]

[ 7, 8, 8, 0, 7, 0, 0, 7, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "Purification", "and", "cloning", "of", "this", "protein", ",", "termed", "early", "B-cell", "factor", "(", "EBF", ")", ",", "from", "murine", "B", "lymphocytes", "and", "independent", "cloning", "of", "a", "protein", ",", "termed", "Olf-1", ",", "from", "olfactory", "neuronal", "cells", "revealed", "virtual", "complete", "amino", "acid", "sequence", "identity", "between", "these", "proteins", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 9, 10, 10, 0, 9, 0, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 7, 8, 8, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "As", "a", "first", "step", "towards", "identifying", "a", "human", "genetic", "disorder", "or", "mouse", "mutation", "for", "which", "EBF", "could", "be", "a", "candidate", "gene", ",", "we", "have", "chromosomally", "mapped", "the", "corresponding", "locus", "in", "both", "species", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 9, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]

[ "By", "Southern", "hybridization", "analyses", "of", "somatic", "cell", "hybrid", "panels", "with", "murine", "cDNA", "probe", ",", "fluorescence", "chromosomal", "in", "situ", "hybridization", "(", "FISH", ")", "of", "human", "genomic", "clones", ",", "and", "analysis", "of", "recombinant", "inbred", "mouse", "strains", ",", "we", "have", "found", "single", "sites", "for", "EBF", "homologous", "sequences", "on", "human", "Chromosome", "(", "Chr", ")", "5", ",", "band", "q34", ",", "and", "on", "proximal", "mouse", "Chr", "11", ",", "in", "an", "evolutionarily", "conserved", "region", "." ]

[ 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 1, 2, 2, 0, 1, 2, 2, 2, 2, 2, 0, 0, 1, 0, 0, 0, 0, 1, 2, 2, 0, 0, 0, 0, 0, 0, 0 ]

[ "Calcineurin", "activates", "transcription", "from", "the", "GM-CSF", "promoter", "in", "synergy", "with", "either", "protein", "kinase", "C", "or", "NF-kappa", "B", "/AP-1", "in", "T", "cells", "." ]

[ 9, 0, 0, 0, 0, 1, 2, 0, 0, 0, 0, 9, 10, 10, 0, 9, 10, 10, 0, 7, 8, 0 ]

[ "Two", "cis-acting", "elements", "GM-kappa", "B/GC-box", "and", "CLE0", ",", "of", "the", "granulocyte-macrophage", "colony-stimulating", "factor", "(", "GM-CSF", ")", "gene", "are", "required", "for", "maximal", "induction", "in", "Jurkat", "T", "cells", "by", "costimulation", "with", "phorbol-12-myristate", "acetate", "(", "PMA", ")", "and", "Ca2+", "ionophore", "(", "A23187", ")", "." ]

[ 0, 0, 0, 1, 2, 0, 1, 0, 0, 0, 1, 2, 2, 2, 2, 2, 2, 0, 0, 0, 0, 0, 0, 5, 6, 6, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0 ]