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17701
[ "Nuclear", "factor", "of", "activated", "T", "cells", "(", "NFAT", ")", "regulates", "transcription", "of", "a", "number", "of", "cytokine", "genes", ",", "and", "NFAT", "DNA", "binding", "activity", "is", "stimulated", "following", "T", "cell", "activation", "." ]
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17702
[ "Several", "lines", "of", "evidence", "have", "suggested", "that", "NFAT", "is", "a", "substrate", "for", "calcineurin", ",", "a", "serine/threonine", "phosphatase", "." ]
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17703
[ "Using", "a", "polyclonal", "antibody", "to", "murine", "NFATp", ",", "Western", "blot", "analysis", "of", "various", "mouse", "tissues", "demonstrated", "that", "the", "110-130-kDa", "NFATp", "protein", "was", "highly", "expressed", "in", "thymus", "and", "spleen", "." ]
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17704
[ "Treatment", "of", "immunoprecipitated", "NFATp", "from", "untreated", "HT-2", "cells", "with", "calcineurin", "resulted", "in", "the", "dephosphorylation", "of", "NFATp", ",", "demonstrating", "that", "NFATp", "is", "an", "in", "vitro", "substrate", "for", "calcineurin", "." ]
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17705
[ "NFATp", "immunoprecipitated", "from", "32P-labeled", "HT-2", "cells", "migrated", "as", "an", "approximately", "120-kDa", "protein", "that", "was", "localized", "to", "the", "cytosol", "of", "the", "cells", "." ]
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17706
[ "Treatment", "of", "the", "cells", "with", "ionomycin", "resulted", "in", "a", "decrease", "in", "the", "molecular", "weight", "of", "NFATp", "and", "a", "loss", "of", "32P", ",", "consistent", "with", "NFATp", "dephosphorylation", "." ]
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17707
[ "The", "dephosphorylation", "of", "NFATp", "was", "accompanied", "by", "localization", "of", "the", "protein", "to", "the", "nuclear", "fraction", "." ]
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17708
[ "Both", "of", "these", "events", "were", "blocked", "by", "preincubation", "of", "the", "cells", "with", "FK506", ",", "a", "calcineurin", "inhibitor", ",", "consistent", "with", "the", "hypothesis", "that", "NFATp", "is", "a", "calcineurin", "substrate", "in", "cells", "." ]
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17709
[ "Activation", "and", "expression", "of", "the", "nuclear", "factors", "of", "activated", "T", "cells", ",", "NFATp", "and", "NFATc", ",", "in", "human", "natural", "killer", "cells", ":", "regulation", "upon", "CD16", "ligand", "binding", "." ]
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17710
[ "The", "putative", "factors", "that", "couple", "the", "signal", "transduction", "from", "surface", "receptors", "to", "the", "activation", "of", "cytokine", "synthesis", "in", "natural", "killer", "(", "NK", ")", "cells", "have", "not", "been", "elucidated", "." ]
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17711
[ "We", "report", "here", "that", "the", "nuclear", "factor", "of", "activated", "T", "cells", "(", "NFATp", ")", ",", "a", "cyclosporin", "A", "(", "CsA", ")", "-sensitive", "factor", "that", "regulates", "the", "transcription", "of", "several", "cytokines", ",", "mediates", "CD16", "-induced", "activation", "of", "cytokine", "genes", "in", "human", "NK", "cells", "." ]
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17712
[ "CD16", "(", "Fc", "gamma", "RIIIA", ")", "-induced", "expression", "of", "cytokine", "mRNA", "in", "NK", "cells", "occurs", "via", "a", "CsA-sensitive", "and", "Ca", "(", "2+", ")", "-dependent", "mechanism", "." ]
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17713
[ "Stimulation", "of", "NK", "cells", "with", "CD16", "ligands", "induces", "NFAT", "-like", "DNA", "binding", "activity", "in", "the", "nuclear", "extracts", "from", "these", "cells", ",", "as", "detected", "in", "electrophoretic", "mobility", "shift", "assays", "." ]
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17714
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17715
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17716
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17717
[ "The", "results", "of", "supershift", "assays", "using", "NFATp-", "and", "NFATc-", "specific", "antibodies", "indicate", "that", "NK", "cell", "activation", "early", "after", "CD16", "ligand", "binding", "involves", "primarily", ",", "if", "not", "exclusively", ",", "NFATp", ",", "and", "Western", "blot", "analysis", "shows", "that", "this", "has", "the", "same", "electrophoretic", "mobility", "(", "approximately", "120", "kD", ")", "as", "that", "of", "T", "lymphocytes", "." ]
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17718
[ "NK", "cells", "do", "not", "express", "NFATc", "constitutively", ",", "but", "NFATc", "mRNA", "accumulation", "is", "induced", "in", "these", "cells", "within", "2", "h", "of", "stimulation", "with", "CD16", "ligands", "." ]
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17719
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17720
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17721
[ "Interleukin", "2", "signaling", "involves", "the", "phosphorylation", "of", "Stat", "proteins", "." ]
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17722
[ "One", "of", "the", "most", "important", "cytokines", "involved", "in", "immune", "response", "regulation", "is", "interleukin", "2", "(", "IL-2", ")", ",", "a", "potent", "activator", "of", "the", "proliferation", "and", "function", "of", "T", "lymphocytes", "and", "natural", "killer", "cells", "." ]
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17723
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17724
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17725
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17726
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17727
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17728
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17729
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17730
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17731
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17732
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17733
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17734
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17735
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17736
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17737
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17738
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17739
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17740
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17741
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17742
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17743
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17744
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17745
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17746
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17747
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