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17801
[ "In", "the", "first", "patients", "studied", ",", "we", "found", "no", "significant", "modification", "in", "the", "expression", "of", "the", "97", "kDa", "Ca", "(", "2+", ")", "-ATPase", "by", "Western", "blotting", "using", "the", "PL/IM", "430", "monoclonal", "antibody", "which", "specifically", "recognized", "this", "isoform", "." ]
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17802
[ "In", "contrast", ",", "the", "Rap1", "protein", "was", "differentially", "phosphorylated", "when", "using", "15", "micrograms/ml", "of", "the", "C.", "Sub", "." ]
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17803
[ "These", "results", "allowed", "us", "to", "use", "these", "pathological", "platelets", "to", "study", "the", "relationship", "between", "the", "expression", "of", "Rap1", "protein", "and", "the", "regulation", "of", "Ca2+", "transport", "by", "selecting", "a", "patient", "with", "severe", "heart", "failure", "." ]
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17804
[ "We", "could", "show", "a", "decrease", "in", "the", "expression", "as", "well", "as", "in", "the", "phosphorylation", "of", "Rap1", "protein", "and", "demonstrate", "a", "lower", "effect", "of", "C.", "Sub", ".", "on", "Ca2+", "transport", "." ]
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17805
[ "Finally", ",", "by", "studying", "a", "further", "series", "of", "patients", ",", "we", "could", "confirm", "that", "the", "decrease", "in", "Rap1", "protein", "expression", "in", "heart", "failure", ",", "whatever", "its", "extent", ",", "was", "variable", ",", "and", "could", "strictly", "correlate", "the", "expression", "of", "Rap1", "protein", "with", "the", "stimulatory", "effect", "of", "C.", "Sub", ".", "on", "Ca2+", "transport", "." ]
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17806
[ "Besides", "the", "evidence", "for", "regulation", "of", "the", "expression", "of", "the", "Rap1", "protein", "in", "platelets", "from", "patients", "with", "heart", "failure", ",", "these", "findings", "constitute", "a", "new", "approach", "in", "favour", "of", "the", "regulation", "of", "platelet", "Ca2+", "transport", "through", "the", "phosphorylation", "of", "the", "Rap1", "protein", "." ]
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17807
[ "An", "IRF-1", "-dependent", "pathway", "of", "DNA", "damage-induced", "apoptosis", "in", "mitogen-activated", "T", "lymphocytes", "." ]
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17808
[ "Lymphocytes", "are", "particularly", "susceptible", "to", "DNA", "damage-induced", "apoptosis", ",", "a", "response", "which", "may", "serve", "as", "a", "form", "of", "'altruistic", "suicide", "'", "to", "counter", "their", "intrinsic", "high", "potential", "for", "mutation", "and", "clonal", "expansion", "." ]
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17809
[ "The", "tumour", "suppressor", "p53", "has", "been", "shown", "to", "regulate", "this", "type", "of", "apoptosis", "in", "thymocytes", ",", "but", "an", "as", "yet", "unknown", ",", "p53", "-independent", "pathway", "(", "s", ")", "appears", "to", "mediate", "the", "same", "event", "in", "mitogen-activated", "mature", "T", "lymphocytes", "." ]
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17810
[ "Here", "we", "show", "DNA", "damage-induced", "apoptosis", "in", "these", "T", "lymphocytes", "is", "dependent", "on", "the", "antioncogenic", "transcription", "factor", "interferon", "regulatory", "factor", "(", "IRF", ")", "-1", "." ]
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17811
[ "Thus", "two", "different", "anti-onco-genic", "transcription", "factors", ",", "p53", "and", "IRF-1", ",", "are", "required", "for", "distinct", "apoptotic", "pathways", "in", "T", "lymphocytes", "." ]
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17812
[ "We", "also", "show", "that", "mitogen", "induction", "of", "the", "interleukin-1", "beta", "converting", "enzyme", "(", "ICE", ")", "gene", ",", "a", "mammalian", "homologue", "of", "the", "Caenorhabditis", "elegans", "cell", "death", "gene", "ced-3", ",", "is", "IRF-1", "-dependent", "." ]
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17813
[ "Ectopic", "overexpression", "of", "IRF-1", "results", "in", "the", "activation", "of", "the", "endogenous", "gene", "for", "ICE", "and", "enhances", "the", "sensitivity", "of", "cells", "to", "radiation-induced", "apoptosis", "." ]
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17814
[ "Circadian", "rhythm", "of", "glucocorticoid", "receptors", "in", "human", "peripheral", "leukocytes", "and", "their", "reactivity", "to", "glucocorticoids", "." ]
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17815
[ "1", ")", "There", "exists", "a", "CR", "of", "GR", "in", "human", "leukocytes", ",", "PMN", ",", "and", "monocytes", "with", "the", "peak", "values", "from", "0400", "to", "0800", "hr", "and", "the", "trough", "values", "between", "2300", "and", "0000", "hr", "." ]
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17816
[ "The", "difference", "between", "them", "was", "significant", "statistically", "." ]
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17817
[ "2", ")", "The", "FI", "of", "the", "chemotactic", "migration", "rate", "of", "PMN", "by", "cortisol", "also", "showed", "diurnal", "changes", "which", "were", "synchronous", "with", "that", "of", "GR", "." ]
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17818
[ "This", "indicates", "that", "the", "CR", "of", "GR", "may", "be", "of", "functional", "significance", "." ]
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17819
[ "3", ")", "In", "Cushing", "'s", "syndrome", ",", "the", "CR", "of", "GR", "was", "normal", "in", "spite", "of", "the", "fact", "that", "the", "CR", "of", "plasma", "cortisol", "was", "disturbed", "." ]
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17820
[ "This", "indicates", "the", "independency", "of", "the", "CR", "of", "GR", "from", "that", "of", "cortisol", "." ]
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17821
[ "4", ")", "In", "apoplexy", "caused", "by", "brain", "ischemia", ",", "the", "CR", "of", "GR", "was", "abolished", "in", "patients", "with", "basal", "lesions", "but", "preserved", "when", "the", "lesions", "were", "located", "in", "the", "cerebral", "cortex", "." ]
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17822
[ "These", "results", "strongly", "suggest", "that", "the", "main", "``", "circadian", "pacemaker", "''", "of", "GR", "is", "located", "in", "the", "basal", "brain", ",", "most", "probably", "in", "the", "suprachiasmatic", "nuclei", "as", "has", "been", "suggested", "for", "rodents", "." ]
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17823
[ "B-lymphoblastoid", "cell", "lines", "from", "multiple", "sclerosis", "patients", "and", "a", "healthy", "control", "producing", "a", "putative", "new", "human", "retrovirus", "and", "Epstein-Barr", "virus", "." ]
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17824
[ "On", "several", "occasions", "we", "have", "observed", "retrovirus-like", "particles", "(", "RVLPs", ")", "by", "transmission", "electron", "microscopy", "(", "EM", ")", "of", "cultured", "T", "cells", "from", "a", "patient", "with", "MS", "." ]
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17825
[ "Later", "we", "established", "spontaneously", "formed", "B-lymphoblastoid", "cell", "lines", "(", "LCLs", ")", "from", "a", "patient", "with", "an", "MS-like", "disease", "and", "from", "another", "patient", "with", "MS", "who", "had", "a", "reactivated", "Epstein-Barr", "virus", "(", "EBV", ")", "infection", "." ]
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17826
[ "Both", "LCLs", "were", "found", "by", "EM", "to", "produce", "RVLP", "and", "EBV", "particles", "." ]
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17827
[ "Reverse", "transcriptase", "(", "RT", ")", "assays", "were", "positive", "in", "purified", "viral", "material", "from", "both", "LCLs", "." ]
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17828
[ "To", "substantiate", "these", "findings", "we", "initiated", "an", "intensified", "culturing", "procedure", "and", "were", "able", "to", "establish", "LCLs", "from", "5", "out", "of", "21", "consecutive", "MS", "patients", "and", "1", "out", "of", "13", "consecutive", "healthy", "controls", "." ]
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17829
[ "All", "LCLs", "were", "found", "to", "produce", "both", "RVLP", "and", "EBV", "particles", "by", "EM", "." ]
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17830
[ "Whether", "the", "putative", "new", "retrovirus", "(", "es", ")", "and", "EBV", "have", "any", "causal", "relationship", "to", "MS", "is", "still", "not", "known", ",", "but", "the", "findings", "support", "this", "possibility", "." ]
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17831
[ "Identification", "of", "an", "ionomycin/cyclosporin", "A-responsive", "element", "within", "the", "human", "T", "cell", "receptor", "gamma", "enhancer", "." ]
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17832
[ "Activation", "through", "the", "Ca2+/", "calcineurin", "pathway", "is", "essential", "to", "the", "transcription", "of", "many", "cytokine", "genes", "." ]
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17833
[ "The", "conserved", "cis-acting", "sequence", ",", "GGAAAA", ",", "and", "transcription", "factors", "binding", "to", "this", "sequence", "are", "involved", "in", "the", "response", "to", "increased", "intracellular", "Ca2+", "concentrations", "." ]
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17834
[ "Here", "we", "report", "the", "identification", "and", "importance", "of", "the", "same", "sequence", "in", "a", "non-cytokine", "gene", ",", "the", "human", "T", "cell", "receptor", "gamma", "(", "TCRG", ")", "enhancer", "." ]
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17835
[ "Results", "from", "site-directed", "mutations", "and", "electrophoretic", "mobility", "shift", "assays", "strongly", "suggest", "that", "this", "sequence", "mediates", "the", "ionomycin-induced", "activation", "of", "the", "TCRG", "enhancer", "." ]
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17836
[ "Our", "studies", "provide", "an", "explanation", "for", "a", "previous", "observation", "that", "TCRG", "mRNA", "levels", ",", "but", "not", "mRNA", "levels", "for", "T", "cell", "receptor", "alpha", "and", "-beta", ",", "are", "increased", "by", "ionomycin", "treatment", "." ]
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17837
[ "Coexpression", "of", "NF-kappa", "B/Rel", "and", "Sp1", "transcription", "factors", "in", "human", "immunodeficiency", "virus", "1-induced", ",", "dendritic", "cell-T-cell", "syncytia", "." ]
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17838
[ "Productive", "infection", "of", "T", "cells", "with", "human", "immunodeficiency", "virus", "1", "(", "HIV-1", ")", "typically", "requires", "that", "the", "T", "cells", "be", "stimulated", "with", "antigens", "or", "mitogens", "." ]
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17839
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17840
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17841
[ "These", "syncytia", "lack", "activated", "cells", "as", "determined", "by", "an", "absence", "of", "staining", "for", "Ki-67", "cell", "cycle", "antigen", "." ]
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17842
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17843
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17844
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17845
[ "DCs", "express", "high", "levels", "of", "all", "known", "NF-kappa", "B", "and", "Rel", "proteins", ",", "with", "activity", "residing", "primarily", "within", "RelB", ",", "p50", ",", "and", "p65", "." ]
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17846
[ "However", ",", "DCs", "lack", "Sp1", ",", "which", "may", "explain", "the", "failure", "of", "HIV-1", "to", "replicate", "in", "purified", "DCs", "." ]
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17847
[ "Coexpression", "of", "NF-kappa", "B", "and", "Sp1", "occurs", "in", "the", "heterologous", "DC-T-cell", "syncytia", "that", "are", "induced", "by", "HIV-1", "." ]
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17848
[ "Therefore", ",", "HIV-1-induced", "cell", "fusion", "brings", "together", "factors", "that", "upregulate", "virus", "transcription", "." ]
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17849
[ "Since", "DCs", "and", "memory", "T", "cells", "frequently", "traffic", "together", "in", "situ", ",", "these", "unusual", "heterologous", "syncytia", "could", "develop", "in", "infected", "individuals", "and", "lead", "to", "chronic", "HIV-1", "replication", "without", "ostensible", "immune", "stimulation", "." ]
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17850
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17851
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17852
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17853
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17854
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17855
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17856
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17857
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17858
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17859
[ "The", "B4-2", "clone", "coded", "for", "an", "mRNA", "of", "2061", "bp", "in", "length", "." ]
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17860
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17861
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17862
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17863
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17864
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17865
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17866
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17867
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17868
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17869
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17870
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17871
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17872
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17873
[ "We", "therefore", "propose", "that", "IL-2", "receptor", "signal", "transduction", "does", "not", "depend", "on", "equimolar", "heterodimerization", "of", "JAK1", "and", "JAK3", "following", "IL-2", "-induced", "heterodimerization", "of", "IL-2R", "beta", "and", "IL-2R", "gamma", "." ]
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17874
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17875
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17876
[ "Up-regulation", "of", "high-affinity", "dehydroepiandrosterone", "binding", "activity", "by", "dehydroepiandrosterone", "in", "activated", "human", "T", "lymphocytes", "." ]
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17877
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17878
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17879
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17880
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17881
[ "Ubiquitin-mediated", "processing", "of", "NF-kappa", "B", "transcriptional", "activator", "precursor", "p105", "." ]
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17882
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17883
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17884
[ "p50", "is", "translated", "as", "a", "precursor", "of", "105", "kDa", "." ]
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17885
[ "The", "C-terminal", "domain", "of", "the", "precursor", "is", "rapidly", "degraded", ",", "forming", "the", "mature", "p50", "subunit", "consisted", "of", "the", "N-terminal", "region", "of", "the", "molecule", "." ]
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17886
[ "The", "mechanism", "of", "generation", "of", "p50", "is", "not", "known", "." ]
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17887
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17888
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17889
[ "They", "have", "also", "shown", "that", "proteasome", "inhibitors", "block", "the", "processing", "both", "in", "vitro", "and", "in", "vivo", "." ]
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17890
[ "In", "this", "study", ",", "we", "demonstrate", "reconstitution", "of", "a", "cell-free", "processing", "system", "and", "demonstrate", "directly", "that", ":", "(", "a", ")", "the", "ubiquitin", "-proteasome", "system", "is", "involved", "in", "processing", "of", "the", "intact", "p105", "precursor", ",", "(", "b", ")", "conjugation", "of", "ubiquitin", "to", "the", "precursor", "is", "an", "essential", "intermediate", "step", "in", "the", "processing", ",", "(", "c", ")", "the", "recently", "discovered", "novel", "species", "of", "the", "ubiquitin-carrier", "protein", ",", "E2-F1", ",", "that", "is", "involved", "in", "the", "conjugation", "and", "degradation", "of", "p53", ",", "is", "also", "required", "for", "the", "limited", "processing", "of", "the", "p105", "precursor", ",", "and", "(", "d", ")", "a", "novel", ",", "approximately", "320-kDa", "species", "of", "ubiquitin-protein", "ligase", ",", "is", "involved", "in", "the", "process", "." ]
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17891
[ "This", "novel", "enzyme", "is", "distinct", "from", "E6-AP", ",", "the", "p53-conjugating", "ligase", ",", "and", "from", "E3", "alpha", ",", "the", "``", "N-end", "rule", "''", "ligase", "." ]
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17892
[ "Flutamide", "in", "the", "treatment", "of", "hirsutism", ":", "long-term", "clinical", "effects", ",", "endocrine", "changes", ",", "and", "androgen", "receptor", "behavior", "." ]
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17893
[ "OBJECTIVE", ":", "To", "investigate", "the", "long-term", "effects", "of", "treatment", "with", "low", "doses", "of", "flutamide", "on", "clinical", "and", "hormonal", "parameters", ",", "as", "well", "as", "on", "the", "androgen", "receptor", "status", ",", "in", "hirsute", "women", "." ]
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17894
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17895
[ "Barrier", "or", "intrauterine", "contraception", "was", "used", "during", "the", "study", "in", "sexually", "active", "women", "." ]
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17896
[ "Safety", "parameters", "were", "assessed", "throughout", "the", "study", "." ]
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17897
[ "Hirsutism", ",", "graded", "by", "the", "modified", "Ferriman-Gallwey", "score", ",", "and", "hormonal", "parameters", "were", "evaluated", "basally", "and", "at", "4-month", "intervals", "during", "treatment", "." ]
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17898
[ "Gonadotropin-releasing", "hormone", "and", "ACTH", "stimulation", "tests", "were", "performed", "before", "and", "after", "3", "to", "4", "months", "of", "therapy", "." ]
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17899
[ "In", "addition", ",", "the", "concentration", "of", "androgen", "receptors", "in", "mononuclear", "leukocytes", "was", "measured", ",", "in", "both", "the", "follicular", "and", "luteal", "phases", "of", "the", "menstrual", "cycle", ",", "basally", "and", "after", "4", "months", "of", "flutamide", "treatment", "." ]
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17900
[ "RESULTS", ":", "Flutamide", "was", "well", "tolerated", "in", "all", "women", ",", "with", "the", "noticeable", "exception", "of", "one", "patient", "who", "presented", "increased", "serum", "transaminase", "after", "8", "months", "of", "therapy", "." ]
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