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18201
[ "Functional", "characterization", "of", "the", "murine", "homolog", "of", "the", "B", "cell-specific", "coactivator", "BOB.1/OBF.1", "." ]
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18202
[ "B", "cell-specific", "transcriptional", "promoter", "activity", "mediated", "by", "the", "octamer", "motif", "requires", "the", "Oct1", "or", "Oct2", "protein", "and", "additional", "B", "cell-restricted", "cofactors", "." ]
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18203
[ "One", "such", "cofactor", ",", "BOB.1/OBF.1", ",", "was", "recently", "isolated", "from", "human", "B", "cells", "." ]
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18204
[ "Here", ",", "we", "describe", "the", "isolation", "and", "detailed", "characterization", "of", "the", "murine", "homolog", "." ]
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18205
[ "Full-length", "cDNAs", "and", "genomic", "clones", "were", "isolated", ",", "and", "the", "gene", "structure", "was", "determined.", "Comparison", "of", "the", "deduced", "amino", "acids", "shows", "88", "%", "sequence", "identity", "between", "mouse", "and", "human", "BOB.1/OBF.1", "." ]
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18206
[ "The", "NH2-terminal", "126", "amino", "acids", "of", "BOB.1/OBF.1", "are", "both", "essential", "and", "sufficient", "for", "interaction", "with", "the", "POU", "domains", "of", "either", "Oct1", "or", "Oct2", "." ]
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18207
[ "This", "protein-protein", "interaction", "does", "not", "require", "the", "simultaneous", "binding", "of", "Oct", "proteins", "to", "DNA", ",", "and", "high", "resolution", "footprinting", "of", "the", "Oct", "-DNA", "interaction", "reveals", "that", "binding", "of", "BOB.1/OBF.1", "to", "Oct1", "or", "Oct2", "does", "not", "alter", "the", "interaction", "with", "DNA", "." ]
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18208
[ "BOB.1/OBF.1", "can", "efficiently", "activate", "octamer-dependent", "promoters", "in", "fibroblasts", ";", "however", ",", "it", "fails", "to", "stimulate", "octamer-dependent", "enhancer", "activity", "." ]
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18209
[ "Fusion", "of", "subdomains", "of", "BOB.1/OBF.1", "with", "the", "GAL4", "DNA", "binding", "domain", "reveals", "that", "both", "NH2-", "and", "COOH-terminal", "domains", "of", "BOB.1/OBF.1", "contribute", "to", "full", "transactivation", "function", ",", "the", "COOH-terminal", "domain", "is", "more", "efficient", "in", "this", "transactivation", "assay", "." ]
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18210
[ "Consistent", "with", "the", "failure", "of", "full-length", "BOB.1/OBF.1", "to", "stimulate", "octamer-dependent", "enhancer", "elements", "in", "non", "B", "cells", ",", "the", "GAL4", "fusions", "likewise", "only", "stimulate", "from", "a", "promoter-proximal", "position", "." ]
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18211
[ "Anti-immunoglobulin", "M", "activates", "nuclear", "calcium/calmodulin-dependent", "protein", "kinase", "II", "in", "human", "B", "lymphocytes", "." ]
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18212
[ "We", "and", "others", "have", "previously", "shown", "that", "the", "nuclear", "protein", ",", "Ets-1", ",", "is", "phosphorylated", "in", "a", "calcium-dependent", "manner", "after", "ligation", "of", "immunoglobulin", "(", "Ig", ")", "M", "on", "B", "lymphocytes", "." ]
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18213
[ "As", "this", "phosphorylation", "was", "independent", "of", "protein", "kinase", "C", "activity", ",", "we", "tested", "whether", "a", "calcium/calmodulin-dependent", "protein", "kinase", "(", "CaM", "kinase", ")", "might", "phosphorylate", "the", "Ets-1", "protein", "after", "elevation", "of", "intracellular", "free", "calcium", "concentrations", "." ]
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18214
[ "The", "dephosphorylated", "form", "of", "Ets-1", "has", "been", "shown", "to", "bind", "to", "chromatin", ",", "suggesting", "that", "the", "operative", "kinase", "should", "be", "detectable", "in", "the", "nucleus", "." ]
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18215
[ "We", "prepared", "nuclear", "extracts", "from", "two", "human", "B", "cell", "lines", "in", "which", "increased", "intracellular", "free", "calcium", "levels", "correlated", "with", "increased", "phosphorylation", "of", "the", "Ets-1", "protein", "." ]
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18216
[ "Activity", "of", "the", "CaM", "kinases", "was", "determined", "using", "a", "synthetic", "peptide", "substrate", "both", "in", "the", "absence", "and", "presence", "of", "an", "inhibitor", "specific", "for", "the", "CaM", "kinase", "family", ",", "KN-62", "." ]
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18217
[ "Stimulation", "of", "cells", "with", "anti-IgM", "led", "to", "increased", "activity", "of", "a", "nuclear", "kinase", "that", "could", "phosphorylate", "the", "peptide", ",", "and", "this", "activity", "was", "reduced", "by", "10", "microM", "KN-62", "." ]
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18218
[ "Kinase", "activity", "was", "reduced", "in", "lysates", "preadsorbed", "using", "an", "antibody", "specific", "for", "CaM", "kinase", "II", "." ]
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18219
[ "Two-dimensional", "phosphopeptide", "maps", "of", "the", "Ets-1", "protein", "from", "cells", "incubated", "with", "ionomycin", "or", "anti-IgM", "contained", "two", "unique", "phosphopeptides", "that", "were", "absent", "in", "untreated", "cells", "." ]
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18220
[ "Incubation", "of", "isolated", "Ets-1", "protein", "with", "purified", "CaM", "kinase", "II", "produced", "phosphorylation", "of", "peptides", "that", "migrated", "identically", "to", "those", "found", "in", "cells", "incubated", "with", "either", "anti-IgM", "or", "ionomycin", "." ]
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18221
[ "These", "data", "suggest", "a", "model", "of", "signal", "transduction", "by", "the", "antigen", "receptor", "on", "B", "lymphocytes", "in", "which", "increased", "intracellular", "free", "calcium", "can", "rapidly", "activate", "nuclear", "CaM", "kinase", "II", ",", "potentially", "resulting", "in", "phosphorylation", "and", "regulation", "of", "DNA-binding", "proteins", "." ]
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18222
[ "Transcriptional", "activation", "and", "repression", ",", "two", "properties", "of", "the", "lymphoid-specific", "transcription", "factor", "Oct-2a", "." ]
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18223
[ "The", "lymphoid-specific", "transcription", "factor", "Oct-2a", "contains", "two", "transcriptional", "activation", "domains", "which", "are", "located", "within", "the", "N-terminal", "and", "C-terminal", "regions", "." ]
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18224
[ "To", "study", "their", "differential", "activation", "properties", ",", "we", "linked", "the", "isolated", "effector", "domains", "to", "the", "GAL4", "DNA-binding", "domain", "." ]
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18225
[ "We", "have", "shown", "that", "both", "activating", "regions", "of", "Oct-2a", ",", "isolated", "from", "their", "natural", "context", ",", "can", "activate", "transcription", "as", "promoter", "factors", "." ]
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18226
[ "In", "contrast", "to", "the", "C-terminus", ",", "activation", "by", "the", "N-terminal", "domain", "is", "dependent", "on", "a", "yet", "unidentified", "factor", "(", "s", ")", "binding", "to", "the", "simian", "virus", "40", "enhancer", "." ]
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18227
[ "The", "results", "obtained", "by", "duplication", "of", "activation", "domains", "or", "their", "mixed", "combination", "suggest", "that", "the", "domains", "are", "functionally", "independent", "." ]
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18228
[ "However", ",", "activation", "from", "a", "remote", "position", "could", "only", "be", "achieved", "with", "the", "C-terminus", "of", "Oct-2a", "in", "B", "cells", "." ]
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18229
[ "In", "lymphoid", "cells", ",", "higher", "activation", "levels", "were", "observed", ",", "suggesting", "that", "distinct", "B-cell-specific", "cofactors", "in", "concert", "with", "the", "effector", "domains", "of", "Oct-2a", "might", "be", "involved", "in", "mediating", "transcription", "from", "proximal", "and", "remote", "positions", "." ]
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18230
[ "Furthermore", ",", "we", "identified", "a", "repression", "domain", "at", "the", "N-terminus", "of", "Oct-2a", "." ]
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18231
[ "When", "transferred", "to", "a", "potent", "activator", ",", "transcriptional", "stimulation", "was", "inhibited", "efficiently", "." ]
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18232
[ "These", "results", "underscore", "the", "modular", "structure", "of", "Oct-2a", "with", "separable", "domains", "for", "activation", "and", "repression", "and", "suggest", "that", "Oct-2a", "might", "have", "complex", "regulatory", "functions", "in", "vivo", "." ]
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18233
[ "Nonopsonic", "phagocytosis", "of", "Pseudomonas", "aeruginosa", "by", "macrophages", "and", "polymorphonuclear", "leukocytes", "requires", "the", "presence", "of", "the", "bacterial", "flagellum", "." ]
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18234
[ "Whereas", "the", "mechanism", "of", "nonopsonic", "phagocytosis", "of", "Pseudomonas", "aeruginosa", "has", "been", "described", ",", "the", "bacterial", "ligands", "required", "are", "poorly", "understood", "." ]
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18235
[ "To", "identify", "the", "requisite", "bacterial", "ligands", ",", "studies", "with", "isogenic", "mutants", "of", "P.", "aeruginosa", "PAK", "lacking", "pili", ",", "flagella", ",", "and", "the", "RpoN", "sigma", "factor", "were", "undertaken", "." ]
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18236
[ "The", "RpoN", "mutant", ",", "lacking", "pili", ",", "flagella", ",", "and", "nonpilus", "adhesins", ",", "bound", "poorly", "and", "was", "resistant", "to", "ingestion", "by", "both", "macrophages", "and", "neutrophils", "." ]
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18237
[ "Pili", "were", "not", "absolutely", "required", "for", "binding", "or", "phagocytosis", "of", "P.", "aeruginosa", "." ]
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18238
[ "The", "presence", "of", "a", "flagellum", "was", "not", "required", "for", "binding", "of", "P.", "aeruginosa", "to", "macrophages", "but", "was", "critical", "for", "the", "subsequent", "internalization", "of", "the", "bacterium", ",", "suggesting", "that", "this", "factor", "or", "a", "surface", "ligand", "associated", "with", "its", "assembly", "was", "responsible", "for", "stimulation", "of", "nonopsonic", "phagocytosis", "." ]
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18239
[ "Identification", "of", "essential", "GATA", "and", "Ets", "binding", "motifs", "within", "the", "promoter", "of", "the", "platelet", "glycoprotein", "Ib", "alpha", "gene", "." ]
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18240
[ "Platelet", "glycoprotein", "(", "GP", ")", "Ib-IX-V", "is", "a", "multisubunit", "adhesion", "receptor", "that", "supports", "platelet", "attachment", "to", "thrombogenic", "surfaces", "at", "sites", "of", "vascular", "injury", "." ]
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18241
[ "The", "congenital", "absence", "of", "the", "receptor", "results", "in", "a", "bleeding", "disorder", "associated", "with", "``", "giant", "''", "platelets", ",", "a", "condition", "linking", "the", "expression", "of", "the", "complex", "to", "platelet", "morphogenesis", "." ]
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18242
[ "To", "understand", "better", "the", "expression", "of", "the", "GP", "Ib-IX-V", "complex", ",", "studies", "were", "undertaken", "to", "define", "the", "essential", "genetic", "elements", "supporting", "the", "expression", "of", "the", "alpha-subunit", "of", "the", "complex", "(", "GP", "Ib", "alpha", ")", "." ]
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18243
[ "GP", "Ib", "alpha", "promoter", "activity", "was", "evaluated", "by", "transfection", "of", "human", "erythroleukemia", "cells", "with", "reporter", "plasmids", "coding", "for", "the", "enzyme", ",", "luciferase", "." ]
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18244
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18245
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18246
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18247
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18248
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18249
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18250
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18251
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18252
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18253
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18254
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18255
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[ "Pathogenesis", "of", "atherosclerosis", "." ]
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18257
[ "The", "earliest", "lesion", "in", "the", "development", "of", "an", "atherosclerotic", "plaque", "is", "the", "fatty", "streak", "." ]
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18258
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18260
[ "At", "least", "1", "major", "gene", "modulates", "the", "oxidation", "of", "LDL", "lipids", "and/or", "the", "biologic", "response", "to", "these", "lipids", "." ]
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18261
[ "The", "inverse", "relation", "between", "high", "density", "lipoprotein", "(", "HDL", ")", "and", "atherosclerotic", "events", "may", "in", "part", "be", "due", "to", "enzymes", "associated", "with", "HDL", "that", "destroy", "the", "biologically", "active", "lipids", "generated", "in", "LDL", "." ]
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18262
[ "Estrogen", "and", "progesterone", "receptors", "in", "vernal", "keratoconjunctivitis", "." ]
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18263
[ "PURPOSE", ":", "Sex-related", "influences", "have", "been", "implicated", "in", "the", "pathogenesis", "of", "vernal", "keratoconjunctivitis", "(", "VKC", ")", ",", "an", "allergic", "eosinophilic", "disease", "." ]
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18264
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18265
[ "RESULTS", ":", "Both", "the", "epithelium", "and", "subepithelium", "of", "the", "tarsal", "and", "bulbar", "conjunctiva", "of", "patients", "with", "VKC", ",", "but", "not", "those", "of", "four", "nonatopic", "control", "subjects", ",", "showed", "intense", "positive", "staining", "for", "estrogen", "and", "progesterone", "receptors", "." ]
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18266
[ "Immunofluorescence", "colocalization", "of", "both", "estrogen", "and", "progesterone", "receptors", "with", "eosinophil", "cationic", "protein", "showed", "that", "approximately", "70", "%", "of", "positive", "cells", "were", "eosinophils", "." ]
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18267
[ "CONCLUSIONS", ":", "Sexual", "hormones", ",", "through", "their", "receptors", ",", "may", "influence", "the", "activity", "of", "eosinophils", "in", "patients", "with", "VKC", "." ]
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18268
[ "CIITA", "activates", "the", "expression", "of", "MHC", "class", "II", "genes", "in", "mouse", "T", "cells", "." ]
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18269
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18270
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18271
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18272
[ "The", "expression", "of", "MHC", "class", "II", "genes", "in", "mouse", "T", "cells", "can", "be", "reconstituted", "upon", "transfection", "with", "the", "human", "CIITA", "cDNA", "." ]
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18273
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18274
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18275
[ "Several", "of", "the", "heterogeneous", "clinical", "manifestations", "of", "systemic", "lupus", "erythematosus", "have", "been", "associated", "with", "specific", "autoantibodies", "." ]
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18276
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18277
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18278
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18279
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18280
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18281
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18282
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18283
[ "Activation", "of", "individual", "CD4+", "T", "cells", "results", "in", "differential", "lymphokine", "expression", ":", "interleukin", "2", "(", "IL-2", ")", "is", "preferentially", "produced", "by", "T", "helper", "type", "1", "(", "TH1", ")", "cells", ",", "which", "are", "involved", "in", "cell-mediated", "immune", "responses", ",", "whereas", "IL-4", "is", "synthesized", "by", "TH2", "cells", ",", "which", "are", "essential", "for", "humoral", "immunity", "." ]
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18284
[ "The", "Ca", "(", "2+", ")", "-dependent", "factor", "NF-ATp", "plays", "a", "key", "role", "in", "the", "inducible", "transcription", "of", "both", "these", "lymphokine", "genes", "." ]
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18285
[ "However", ",", "while", "IL2", "expression", "requires", "the", "contribution", "of", "Ca", "(", "2+", ")", "-", "and", "protein", "kinase", "C", "-dependent", "signals", ",", "we", "report", "that", "activation", "of", "human", "IL4", "transcription", "through", "the", "Ca", "(", "2+", ")", "-dependent", "pathway", "is", "diminished", "by", "protein", "kinase", "C", "stimulation", "in", "Jurkat", "T", "cells", "." ]
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18286
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18287
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18288
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18289
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18290
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18291
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18292
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18293
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18294
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18295
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18296
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18297
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18298
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18299
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18300
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